Ninty-two accessions of ‘Shazikongxinli’ germplasm with elite traits were selected by visiting and surveying to evaluate the genetic diversity of Prunus salicina Lindl. ‘Shazikongxinli’ IRAP primers were developed based on the conserved regions of RT sequences of plum reverse transcription transposons Ty1-copia and Ty3-gypsy. L₁₆（4³） orthogonal experiments were designed to optimize the main factors affecting content of IRAP-PCR with 10 μL volume. 18 IRAP primers were used to analyze the genetic diversity and construct the fingerprint of the germplasm tested. The results showed that the optimized 10 μL IRAP-PCR reaction system was 1.3 μL of 5-10 mol/L IRAP primers， 5.0 μL of PCR mixture， 1.0 μL of template DNA（30 ng/μL）， and the number of PCR cycles was 40 times. A total of 189 loci were amplified from 18 IRAP primers with good repeatability and high polymorphism， including 180 polymorphic loci.The average value of observed number of alleles（N_a）， effective number of alleles（N_e）， Nei’s gene diversity（H_e） and Shannon’s information index（I） for all primers was 1.930，1.426，0.261 and 0.405， respectively. The 92 accessions tested were clustered into 4 groups using the neighbor-joining method. The IRAP primers Ty3-2 and Ty3-6 were identified as core primers， which can be used to efficiently construct the fingerprints of 92 accessions tested.