At present,the method of cutting potato tissue culture seedling is still using scissors to cut. This method has low efficiency of production and is easy to cause virus infection,which makes it difficult to control the quality of transplanting seedling. To solve the problems mentioned above,using laser to cut potato tissue culture seedlings without contact was proposed. The growth of potato tissue culture seedlings after laser cutting and mechanical shearing was compared. The experimental group used CO2laser to cut potato tissue culture seedlings under the conditions of vertical irradiation,defocus of 12 mm and light output power of 10 W,while the control group used mechanical shearing. Results showed that potato tissue culture seedlings cut with laser did not affect its biological activity. After 21 days of culture,the survival rate,plant height,number of internodes,internode length,stem diameter,root length and fresh weight of root removal of laser cutting were better than those of mechanical shearing for both single-incision and double-incision stems. It is indicated that the non-contact cutting of potato tissue culture seedlings by laser cutting instead of traditional mechanical shearing greatly simplifies the transplantation of tissue culture seedlings,effectively avoids cross-infection of the tissue culture seedlings,and promotes the stem survival of transplanted tissue culture seedlings.