Isolation,identification and susceptibility analysis of pathogenic Klebsiella pneumonia from diseased Procambarus clarkii
DOI:
Author:
Affiliation:

Clc Number:

Fund Project:

  • Article
  • |
  • Figures
  • |
  • Metrics
  • |
  • Reference
  • |
  • Related
  • |
  • Cited by
  • |
  • Materials
  • |
  • Comments
    Abstract:

    A pathogenic bacterium was isolated from the diseased Procambarus clarkiin Qianjiang,Hubei Province.The strain was identified by physiological and biochemical assay,16S rRNA sequencing and phylogenetic analysis.Then susceptibility of the strain to antibiotics was performed by the agar diffusion method,and the minimum inhibitory concentrations (MICs) were evaluated by the microdilution method.The results showed that the physiological and biochemical characteristics of the isolated strain were similar to that of Klebsiella pheumonia.The 16S rRNA sequencing and phylogenetic analysis showed that the 16S rRNA sequence of the strain shared over 99% similarities with that of K. pheumonia.Therefore,the strain was identified as K.pheumonia.According to the susceptibility results,the strain was sensitive to cefotaxime and polymyxin B among all the tested antibiotics.These results above indicated that the strain was a multiresistant K. pheumonia strain.

    Reference
    Related
    Cited by
Get Citation

董靖,张露珊,刘绍春,张国栋,周顺,杨秋红,艾晓辉. Isolation, identification and susceptibility analysis of pathogenic Klebsiella pneumonia from diseased Procambarus clarkii[J]. Jorunal of Huazhong Agricultural University,2021,40(1):93-98.

Copy
Share
Article Metrics
  • Abstract:
  • PDF:
  • HTML:
  • Cited by:
History
  • Received:June 08,2020
  • Revised:
  • Adopted:
  • Online: February 10,2021
  • Published: