Using 49 filamentous fungi with whole genome sequence published as the research objects,OrthoVenn2 homologous gene cluster alignment,BLASTp comparison,keyword search and SMART were used to analyze the homologous gene and conservative domain of regulators of G-protein signaling (RGS). The results showed that there were 229 RGS in the 49 fungi,the number of RGS in each fungus ranged from 3 to 9,and the number of RGS of the necrotrophs and hemibiotrophs fungi was higher than that of the biotrophs fungi. According to the conserve domains,the RGS proteins could be divided into six types,including DEP-RGS,RGS-TM,PXA-RGS-PX,RGS,RGS-PAS-PAC,and TM-RGS,of which RGS with two special conserved domains,RGS-PAS-PAC and TM-RGS,were mainly concentrated in the hemibiotrophs and necrotrophs fungi. Further genetic analysis showed that RGS proteins with the same conserved domain were closely related. The above results indicated that there was a certain correlation between the parasitism and the quantity and species of RGS protein in plant pathogenic filamentous fungi.