Establishment of molecular ID for pear cultirars based on SSR markers
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    Abstract:

    Seventeen pairs of SSR primers with clear polymorphic bands were screened from 60 pairs of SSR locus covering 17 linkage groups of pear genetic map and amplified with 20 pear cultivars.136 polymorphic bands in total were obtained with 5~11 bands per primer locus (8.0 bands on average).The polymorphism information content (PIC) of the 17 SSR loci ranged from 0.614 to 0.848 with an average of 0.733.All pear cultivars studied could be identified with 38 different combinations of 2 markers each.Bands amplified by each marker were coded based on size of fragment and used as a molecular ID.

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张靖国,田瑞,陈启亮,杨晓平,胡红菊. Establishment of molecular ID for pear cultirars based on SSR markers[J]. Jorunal of Huazhong Agricultural University,2014,33(01):12-17.

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  • Received:February 21,2013
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