To explore the rational input of ammonia nitrogen load in pond industrial aquaculture, this study investigated the effects of three ammonia nitrogen load inputs on nitrogen removal performance in grass-type aquaculture water bodies using vallisneria natans as the research object.The results showed that: The results showed that:Under the condition of low ammonia nitrogen load, the nitrogen removal rates of both the Vallisneria natans group and the control group could reach 90%. Under the condition of medium ammonia nitrogen load, the nitrogen removal capacities of the Vallisneria natans group and the control group decreased. The total nitrogen removal rate of the Vallisneria natans group (70.00±0.42%) was slightly higher than that of the control group (64.66±0.27%). Ammonium nitrogen accumulated in the overlying water of both groups, but the concentration of ammonium nitrogen in the Vallisneria natans group was lower. Under the condition of high ammonia nitrogen load, the total nitrogen removal rates of the Vallisneria natans group and the blank group decreased to 33.63±0.37% and 33.17±0.23%, respectively. The ammonium nitrogen concentration in the overlying water of both groups increased rapidly, with no significant difference between them. However, under all nitrogen load conditions, the concentration of ammonium nitrogen in the sediment interstitial water of the Vallisneria natans group was lower than that of the control group. The calculation results of the contribution of Vallisneria natans to nitrogen removal indicated that under low and medium ammonia nitrogen loads, Vallisneria natans had a certain absorption capacity for pollutants, while high ammonia nitrogen load exerted a negative impact on nitrogen removal by Vallisneria natans. In conclusion, controlling the exogenous ammonia nitrogen load in aquaculture water bodies below the medium concentration (approximately 0.625 kilograms per mu) can ensure the reduction of harmful nitrogen in Vallisneria natans-type aquaculture water bodies.