Abstract:To Understanding the current epidemiological status and dominant strains of calf diarrhea viruses is important for disease prevention and control. In this study, 264 diarrheic calves samples (including 58 anal swabs, 15 intestinal tissues, and 191 feces) from 41 farms in Hubei, Hunan, Henan, Guangxi, Guizhou, Gansu, Shandong, and Inner Mongolia were collected between November 2021 and October 2023. RT-PCR was used for the detection of bovine rotavirus (BRV), bovine coronavirus (BCoV) and bovine viral diarrhea virus (BVDV)BRV, BCoV, and BVDV. Results showed that the individual positive proportion of BRV, BCoV and BVDV were 14.02% (95% CI: 8.44-16.68), 10.23% (95% CI: 6.85-14.53) and 12.12% (95% CI: 10.06-18.80), respectively. The farm positive proportion was 78.05% (95% CI: 62.39-89.44), in which 29.27% (95% CI: 16.13-45.54) had mixed infections. 3.42% (95% CI:1.32-5.88) samples were co-infected with both BRV and BCoV, which was the most commonly combination. Furtherly, the distribution of the above three viruses in different regions, ages, seasons and breeds was analyzed. There was a high risk of BRV infection in the northern China, winter and in calves ≤5 weeks old, and BCoV had a high risk of infection in northern China, and both summer and winter. There was no significantly difference BVDV infection in different regions, ages and breeds. This study determined the current status of BRV, BCoV and BVDV infection and possible risk factors in diarrheic calves in part of China, which provides an important epidemiological basis for the precise prevention and control of diarrhea in calves. Keywords calf diarrhea; BRV; BCoV; BVDV; detection; risk factor analysis