【Objective】To explore the feasibility and the optimal operation parameters combination of spraying foliar fertilizer on Acanthopanax trifoliatus using unmanned aerial vehicle (UAV).【Method】The experiment of spraying foliar fertilizer was carried out by using XAG P20-2019 quadcopter UVA, the three-factor and three-level orthogonal test was designed, spray quality, yield, and blade quality of Acanthopanax trifoliatus were used as indicators, the effects on spray concentration, flight speed and flight height were investigated.【Result】 The results showed that flight speed and flight height had significantly influence on spray quality, while spray concentration, flight speed and flight height had significantly influence on yield and blade quality of Acanthopanax trifoliatus. Comparing and analyzing the optimal operation parameters of spray quality, yield and blade quality of Acanthopanax trifoliatus, it can be obtained that fertilization effect is best when spray concentration is 5 times the recommended concentration, flight speed is 1.0 m×s-1, and flight height is 2.0m, spray quality is the best and compared with the blank control group, yield increased by 27.46%, and chlorophyll content of Acanthopanax trifoliatus increased by 33.23%.【Conclusion】This paper proved that the feasibility of spraying high concentration foliar fertilizer on Acanthopanax trifoliatus using unmanned aerial vehicle (UAV). This study is expected to improve the mechanized operation level of the planting industry of Acanthopanax trifoliatus and promote it, and provide reference for foliar fertilization using the same type of UVA with the popularization and application value.