湖北贝母活性成分和内生菌次级代谢产物研究
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1.湖北省农业科学院中药材研究所/湖北民族大学生物科学与技术学院;2.湖北省农业科学院中药材研究所;3.恩施土家族苗族自治州农业农村局

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湖北省重点研发计划项目“道地药材黄连、湖北贝母新品种培育及关键技术研究”(项目编号:2020BCA059);湖北省现代农业产业技术体系:道地药材提质增效生产技术集成创新与示范(项目编号:HBHZD-ZB-2020-005);湖北省农业科学院中药材研究所 2019 年青年基金项目(项目编号 2019ZYCJJ05)。


Study on active components of Fritillaria hupehensis Hsiao et K. C. Hsia and secondary metabolites of its endophytes
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    摘要:

    为探究湖北贝母不同组织贝母素乙含量、内生菌组成及其次级代谢产物的抑菌活性,采用气相色谱法测定湖北贝母鳞茎、花、叶的贝母素乙含量;分别利用16S rRNA和ITS测序技术鉴定内生细菌和真菌;通过琼脂扩散法、微量二倍稀释法研究内生菌次级代谢产物对10株供试菌的抑菌活性。结果表明:湖北贝母叶中贝母素乙含量最高,达3.99±0.10 mg?g-1,其含量是鳞茎的1.78倍、花的4.24倍;从湖北贝母中分离鉴定了4株内生菌,分别为Pseudomonas sp. BM-X-6、Arthrinium sp. BM-Z-5、Aspergillus sp. BM-Z-1、Aspergillus sp. BM-Z-3;3株内生真菌次级代谢产物抑菌活性均优于Pseudomonas sp. BM-X-6,其中Arthrinium sp. BM-Z-5的次级代谢产物抑菌谱最广,对肺炎克雷伯菌、金黄色葡萄球菌、藤黄微球菌、鲍曼不动杆菌、耐甲氧西林金黄色葡萄球菌有中度抑制活性。

    Abstract:

    We aimed to determine the content of peiminine in different tissues of F. hupehensis and species and secondary metabolites of its endophytes. Gas chromatography (GC) was used to determine the content of peiminine in bulbs, flowers and leaves of F. hupehensis. The 16S rDNA and ITS sequencing were used to identify the species of endophytic bacteria or fungi, respectively. And AGAR diffusion and double dilution method was used to study the antibacterial activity of secondary metabolites of endophytes against 10 strains of tested bacteria. The results were described below. Leaves of F. hupehensis had the highest content of peiminine with 3.99±0.10 mg?g-1, which was 1.78 and 4.24 times as much as bulb and flower respectively. Four strains of endophytes were isolated from F. hupehensis, identified as Pseudomonas sp. BM-X-6, Arthrinium sp. BM-Z-5, Aspergillus sp. BM-Z-1 and Aspergillus sp. BM-Z-3. The secondary metabolites of three endophytic fungi had stronger antibacterial activity than Pseudomonas sp.BM-X-6. Meanwhile Arthrinium sp. BM-Z-5 had the broadest antibacterial spectrum, which showed moderate antibacterial activities against Klebsiella pneumoniae, Staphylococcus aureus, Micrococcus luteus, Acinetobacter baumannii and methicillin-resistant Staphylococcus aureus(MRSA).

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  • 收稿日期:2021-07-28
  • 最后修改日期:2021-09-03
  • 录用日期:2021-09-06
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