氮肥/花生饼肥配比施用对番茄根际土壤及根系内生细菌群落结构的影响
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广西大学

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国家自然科学基金(31860547);广西创新驱动发展专项(桂科AA17204039);广西科技重大专项(AA17204041)


Effects of different ratios of nitrogen / peanut residue compost on soil and endophytic bacterial community structure in root of Tomato
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    摘要:

    设置不施肥(A)、100%氮肥(B)、75 %/25 %的氮肥/花生饼肥(C)、50 %/50 %的氮肥/花生饼肥(D)、25 %/75 %配比的氮肥/花生饼肥(E)、100 %花生饼肥(F) 6组处理,基于高通量测序技术,分析氮肥/花生饼肥不同配比处理对番茄根际土壤及根系内生细菌群落结构的影响,为番茄的平衡施肥提供理论依据,结果表明:门分类水平,与不施肥(A)相比,不同施肥处理的根际土壤优势细菌门分类组成的占比发生了改变;另一方面,单一的氮肥和饼肥处理均导致番茄植株内生细菌优势细菌门的数量减少;属分类水平,与不施肥(A)相比,不同施肥处理改变了番茄根际土壤和根系内生细菌中优势细菌属的组成和占比。B处理或F处理条件下,假单胞菌属(Pseudomonas)、鞘脂菌属(Sphingobium)、泛菌属(Pantoea)、克雷伯氏菌属 (Klebsiella)等优势菌属缺失,不仅不利于营造番茄植株根际土壤微环境健康,而且不利于植株抗性能力的提升;此外,单一的氮肥或饼肥处理,诱导了诸如青枯菌属(Ralstonia)等病原细菌富集,并成为植株根系优势内生细菌属,从而不利于改良番茄植株根际土壤微环境和提高番茄植株抗性;25%氮肥和75%饼肥处理是改良番茄植株根际土壤微环境以及提高番茄植株抗性的最佳施用配比。

    Abstract:

    Sat 6 treatments as respectively:no fertilizer application (A);100% nitrogen fertilizer application (B);75%nitrogen and 25% peanut residue compost application(C);50% nitrogen and 50% peanut residue compost application (D); 25 % nitrogen and 75% peanut residue compost application (E);100 % peanut residue compost application (F). And bacterial community structure under 6 treatments were analyzed using high-throughput sequencing technique. The results showed that: the composition of soil dominant bacteria in rhizosphere at phylum level was not changed, but their ratios were altered by application different fertilizers. In addition, the amount of dominant endophytic bacteria in root of tomato at phylum level was all decreased by application of single nitrogen or peanut residue compost only. At genus level, the composition and ratios of soil bacteria in rhizosphere and endophytic bacteria in root were also altered by application of different fertilizers. In particularly, some dominant bacteria, such as Pseudomonas, Sphingobium, Pantoea and Klebsiella were disappeared in treatments B and F. It suggested that not only soil health in rhizosphere could not be improved, but also crop resilience could be decreased by application single nitrogen or peanut residue compost only. Moreover, some pathogenic bacteria, such as Ralstonia enriched in roots of tomatoes as dominant endophytic bacteria under single nitrogen or peanut residue compost treatment. It also indicated that soil health in rhizosphere and crop resilience could not be improved by application of single nitrogen or peanut residue compost only. 25% nitrogen and 75% peanut residue compost are the best combination for improving soil health in rhizosphere and crop resilience among above 6 treatments.

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  • 收稿日期:2020-11-17
  • 最后修改日期:2021-01-18
  • 录用日期:2021-03-18
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