新梨七号及其亲本果皮挥发性物质的比较分析
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1.华中农业大学园艺林学学院;2.塔里木大学植物科学学院;3.河北农业大学园艺学院;4.新疆农业科学院轮台国家果树资源圃

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兵团南疆重点产业发展支撑计划(2017DB006-2)


Comparison of Peel Volatile profiles among Pyrus sinkiangensis ‘Xinli No. 7’ and Its Parents
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XPCC South Xinjiang Key Industry Support Plan (2017DB006-2)

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    摘要:

    采用顶空固相微萃取法(HS-SPME)结合气相色谱-质谱联用技术(GC-MS)对新疆阿拉尔和河北保定采集商业成熟期的新梨7号(Pyrus sinkiangensis ‘Xinli No. 7’)果皮挥发性物质进行测定,并将其与来自新疆阿拉尔的早酥(P. bretschneideri Rehd ‘Zaosu’,父本)和库尔勒香梨(P. sinkiangensis Yu ‘Korla’,母本)进行挥发性物质谱差异分析。结果表明:所有样品中共检测出119种挥发性成分;分别在新疆和河北的新梨7号及其父母本中检测到55、53、56和100种挥发性物质。两地新梨7号果皮样本中挥发性物质总含量分别是其父本的4.52和2.48倍,母本的0.47和和0.26倍。同时,新梨7号的挥发性物质分别较父母本减少了8和42种物质,但增加了(E)-2-辛烯醛、1-十二烯、柏木脑、2-庚酮、2-癸酮和1,2,4,5-四甲基-苯6种物质。己醛、(E)-2-己烯醛是四者共有的含量最高成分,新梨7号果皮中(E)-2-己烯醛及己醛等7种物质含量偏向于高值亲本,30种物质含量接近低值亲本。总体来看,杂交后代新梨7号的挥发性物质种类较父母本有所减少,但新增了6种;在总含量上高于父本、低于母本;不同产地对新梨7号的挥发性物质谱有影响。

    Abstract:

    The volatiles in the peels of Xinli No.7 collected from Alar, Xinjiang and Baoding, Hebei Province were detected by using HS-SPME-GC-MS, and the volatiles were compared with P. bretschneideri Rehd ‘Zaosu’ (the male parent) and P. sinkiangensis Yu ‘Korla’ (the female parent) collected from Alar. The results showed that a total of 119 volatile compounds were detected in all samples; 55, 53, 56 and 100 volatile compounds were detected in Xinli No. 7 from Xinjiang and Hebei, Zaosu and Korla pear, respectively. The total volatile concentrations in Xinli No. 7 from Xinjiang and Hebei provinces were 4.52 and 2.48 times higher than those of the male parent, while 0.47 and 0.26 times lower than those of the female parent, respectively. Compared with the male and female parents, 8 and 42 volatiles were under detection level in Xinli No.7, respectively; but (E)-2-octenal, 1-dodecene, cedrol, 2-heptanone, 2-decanone and 1,2,4,5-tetramethyl-benzene were newly detected in Xinli No.7. (E)-2-hexenal and hexanal were the dominant compounds in all 4 samples; (E)-2-hexenal, hexanal together with other 5 compounds in peel of Xinli No.7 were close to the high concentrations of parents, while the concentrations of 30 compounds were close to the low concentrations of parents. In total, the total content of volatiles in Xinli No.7 hybrid was higher than that of the male parent, but lower than that of the female parent. Different origin areas had an effect on the volatile profiles of Xinli No.7.

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  • 收稿日期:2020-11-09
  • 最后修改日期:2021-01-31
  • 录用日期:2021-03-03
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