芥菜胞质雄性不育系结荚性状的筛选和鉴定
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1.华中农业大学园艺林学学院/园艺植物生物学教育部重点实验室;2.华中农业大学园艺林学学院

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国家特色蔬菜产业技术体系项目(CARS-24-A-06)


Identification and Screening of Pod-setting Characters of Cytoplasmic Male Sterile Lines of Mustard(Brassica juncea)
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    摘要:

    本研究对59份不同变种芥菜材料(30份细胞质雄性不育系和29份保持系)的结荚性状进行田间调查,统计结荚畸形情况;同时,利用分子标记对30份芥菜雄性不育系材料进行了鉴定;结果显示,有23份是芥菜hau细胞质雄性不育系,7份是芥菜ogu细胞质雄性不育系;29份是芥菜保持系材料。7份芥菜ogu细胞质雄性不育系和5份芥菜hau细胞质雄性不育系的荚表现均为饱满、直立,其他芥菜hau细胞质雄性不育系荚则表现出不同程度的畸形。以结荚正常的芥菜保持系材料作为对照(CK),对芥菜hau细胞质雄性不育系和芥菜ogu细胞质雄性不育系的花药败育和花器官发育进行细胞学观察,发现芥菜ogu细胞质雄性不育系和芥菜hau细胞质雄性不育系(荚正常)花药败育特征为绒毡层提前降解,芥菜hau细胞质雄性不育系(荚畸形)的败育特征为形成雄蕊—雌蕊嵌合体,初步揭示了芥菜雄性不育系结荚畸形性状的细胞学形成机制。

    Abstract:

    The pod-setting characteristics of 59 materials of mustard (Brassica juncea) which included 30 cytoplasmic sterile lines and 29 maintainers were studied using field investigations in this experiment, molecular markers and cytological observation methods. The results from molecular markers methods indicated 23 of the 59 mustard materials are hau cytoplasmic male sterile lines,7 of the 59 mustard materials were ogu cytoplasmic male sterile line. The results from cytological observation methods showed that siliques of the 29 maintainers、7 ogu cytoplasmic male sterile lines and 5 hau cytoplasmic male sterile lines were plump and vertical, while siliques of the 18 hau cytoplasmic male sterile lines were abnormal to different degree. The flower organ development and anther abortion of hau cytoplasmic male sterile line and Ogu cytoplasmic male sterile line in Brassica juncea using cytological observation. The results showed the characteristic of flower abortion of ogu cytoplasmic male sterile line and hau cytoplasmic male sterile line(normal pods) were the tapetum degraded in advance, while that of hau cytoplasmic male sterile line(abnormal pods) was the formation of stamen-pistil chimera. The results of this study revealed the mechanism of the siliques cytological malformation traits of mustard male sterile lines.

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  • 收稿日期:2020-11-05
  • 最后修改日期:2020-11-24
  • 录用日期:2021-01-21
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