Huazhong Agriculture University
在鱼糜生产过程中，为获得色泽洁白、高弹性高品质的鱼糜制品，通常需用大量水来进行漂洗，从而产生了以肌浆蛋白为主的有机废水，其排放到环境中必将造成环境污染和资源的浪费。若能将鱼糜漂洗液中的蛋白质回收利用，将产生极佳的经济价值和环境效益。本研究探讨了酸-壳聚糖絮凝耦合处理对鱼糜漂洗液中肌浆蛋白回收率的影响，结果发现酸处理（pH 3.0）可以显著提高壳聚糖作为单一絮凝剂时的肌浆蛋白质回收率（从35.52%到81.54%）。然后通过单因素实验和Box-Bechnken中心组合设计对絮凝条件进行优化，确定肌浆蛋白的最佳回收工艺为：pH 6.5，壳聚糖用量250 mg·L-1，温度30℃，时间90 min。在此条件下，可以获得85.23%的高蛋白质回收率以及73.32%的COD值去除率。本研究不仅为鱼糜漂洗液中肌浆蛋白的回收利用提供了理论依据，而且为企业鱼糜加工过程中漂洗废水的净化处理提供了技术支持和参考借鉴。
In the surimi production process, in order to obtain white-colored, high-elastic and high-quality surimi products, a large amount of water is usually used for rinsing, resulting in organic wastewater mainly composed of sarcoplasmic protein, which will be discharged into the environment and cause environmental pollution and waste of resources. If the protein in the surimi rinsing wastewater can be recycled, it will produce excellent economic value and environmental benefits. This study explored the effect of acid-chitosan flocculation coupling treatment on the recovery rate of sarcoplasmic protein in surimi rinsing water. The results found that acid treatment (pH 3.0) can significantly increase the recovery rate of sarcoplasmic protein from 35.52% to 81.54% when chitosan is used as a flocculant. Then through single factor experiments and Box-Bechnken central combination design to optimize the flocculation conditions, the optimal flocculation conditions for sarcoplasmic protein is determined as follows: pH 6.5, chitosan additive amount 250 mg·L-1, temperature 30℃, time 90 min, a high recovery rate of protein of 85.23% and the removal rate of COD of 73.32% can be obtained. This research provides not only a theoretical basis for the recovery and utilization of sarcoplasmic protein in surimi rinsing water, but also a technical support and reference for the purification treatment of rinsing wastewater in the processing of surimi.