广陈皮中多甲氧基黄酮提取物对脂多糖诱导小鼠急性肺损伤的保护作用
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华中农业大学食品科学技术学院

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国家自然科学基金项目(面上项目,重点项目,重大项目)


Protective Effect of Polymethoxyflavones in Chenpi on Lipopolysaccharide-induced Acute Lung Injury in Mice
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    摘要:

    采用D101大孔吸附树脂从广陈皮中获得多甲氧基黄酮提取物,高速逆流色谱得到川陈皮素和桔皮素,高效液相色谱进行纯度鉴定,化合物结构经质谱和核磁共振氢谱鉴定。通过鼻腔滴注脂多糖法建立小鼠急性肺损伤模型,以250mg/kg多甲氧基黄酮提取物组和25、50、100mg/kg低中高川陈皮素组连续灌胃7d,通过小鼠肺组织病理学变化、肺损伤评分、肺组织湿/干重比、髓过氧化物酶活性(MPO)、丙二醛含量(MDA)、谷胱甘肽过氧化物酶(GSH-PX)活性和细胞因子表达量等指标评价多甲氧基黄酮提取物和川陈皮素的抗氧化和抗炎作用。研究结果发现,多甲氧基黄酮提取物组和川陈皮素中剂量组能显著缓解肺组织损伤,降低肺组织湿/干重比、MPO活性、MDA含量和促炎细胞因子的含量,提高GSH-PX活性,且川陈皮素中剂量组与10mg/kg地塞米松组对于急性肺损伤小鼠的抗氧化和抗炎效果相近。以上研究结果表明,多甲氧基黄酮提取物和川陈皮素具有抗氧化和抗炎作用,对急性肺损伤具有一定的保护作用。

    Abstract:

    D101 resin was used to obtain polymethoxyflavones extracts from the Chenpi . High-speed countercurrent chromatography was used to obtain nobiletin and tangeretin. The purity was identified by high performance liquid chromatography. The structure was identified by mass spectrometry and 1H NMR. The mice model of acute lung injury was constructed by intranasal instillation of LPS in the exprienment.Continuous gastric gavage with 25, 50, 100mg/kg low , medium,high-nobiletin group and 250mg/kg PMFs group for 7d.The anti-oxidant and anti-inflammation of PMFs and NOB was evaluated by assessing pulmonary histopathological changes,lung injury score,the wet weight to dry weight ratio, MPOactivity, MDA content, GSH-PX activity,the cytokine content of lung tissue.The results showed that the PMFs group and middle-NOB group can significantly relieve lung tissue damage, reduced the wet/dry weight ratio, MPO activity, MDA content and pro-inflammatory cytokine content, increased GSH-PX activity.The anti-oxidation and anti-inflammatory effects of the middle-NOB group and the 10 mg/kg dexamethasone group on acute lung injury mice are similar. The above results indicated that PMFs extracts and NOB have anti-oxidant and anti-inflammatory effects, and have certain protective effects on acute lung injury.

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  • 收稿日期:2020-09-29
  • 最后修改日期:2020-11-23
  • 录用日期:2020-11-25
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