Taking Nemipterus virgatus surimi as the research object, Analysis of transglutamianse of Nemipterus virgatus surimi 3D printing effect, as the transglutamianse, with the increase of adding amount of Nemipterus virgatus surimi slurry's gel strength, elasticity, water, disulfide bond and tanδ content showed a trend of decline after rising first, when transglutamianse addition amount of 0.2% maximum, increased by 70.88% respectively than control group, 25.01% and 9.72% and 61.63%. Hardness, apparent viscosity, yield stress and elastic modulus of surimi increased with the addition of transglutamianse. The immobile water of Nemipterus virgatus surimi reached its maximum when the transglutamianse content was 0.2% the peak ratio of T23 reached the highest of 0.97.When the amount of transglutamianse was 0.2%, the sample's surface precision was high and the set model was relatively matched, and the consistency and adhesion between surimi filaments were high.