大豆与丛枝菌根真菌共生建立及菌根检测方法的探究
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华中农业大学

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国家重点基础研究发展计划(973计划)


Establishment of symbiosis between soybean and arbuscular mycorrhizal (AM) fungi and study of mycorrhizal detection method
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    摘要:

    利用盆栽培养的方法,研究四种培养基质对不同品种大豆与AM真菌(Rhizophagus irregularis)共生的影响,比较parker纯黑墨水和台盼蓝两种染色剂对大豆菌根的染色效果,探究放大网格交叉法和Trouvelot五级分级法在大批量菌根共生指标检测中的应用。结果表明:蛭石和土(V:V=4:1)这种基质更有利于AM真菌对大豆的侵染,该基质中菌根的丛枝丰度、根内菌丝密度、泡囊丰度均显著高于其他基质,可以作为大豆与AM真菌共生盆栽培养的最佳培养基质。对于大批量的菌根共生指标检测来说,墨水染色技术和放大网格交叉法是目前最优的菌根染色和真菌指标检测方法。

    Abstract:

    The effects of four Culture medium on the symbiosis of different varieties of soybean and AM fungi (Rhizophagus irregularis) were studied by pot culture. The difference between black ink and trypan blue staining methods and the application of the magnified grid line and the five-class methods in mycorrhizal symbiosis structures detection were also compared in this research.The results show that vermiculite and soil (V: V = 4: 1) are more conducive to the infection of AM fungi with soybean. The arbuscular abundance, hypha density and vesicle abundance in this culture substrate are significant higher than others, so it can be used as the best culture substrates for soybean and AM fungi symbiosis. For large-scale mycorrhizal symbiosis structures detection, ink staining and the magnified grid line method are the best methods in currently.

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历史
  • 收稿日期:2020-05-10
  • 最后修改日期:2020-06-10
  • 录用日期:2020-09-14
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