National Science and Technology Major Project；National Key Research and Development Program of China during the 13th Five-Year Plan Period；The Fundamental Research Funds for the Central Universities
为探究PGF2α信号对水牛卵母细胞和胚胎发育的作用，利用免疫荧光和荧光定量检测了PGF2α受体（PTGFR）在水牛卵母细胞和胚胎早期发育过程中的表达规律；然后在卵母细胞体外成熟液、胚胎培养发育液中分别添加不同浓度的AL-8810，检测其对水牛卵母细胞成熟效率以及胚胎发育的效率的影响。结果显示，PTGFR蛋白在水牛卵母细胞及胚胎中广泛分布，在4-16-细胞期胚胎阶段，PTGFR基因表达水平显著高于未受精的卵母细胞和桑椹胚（P＜0.01）；AL-8810对水牛卵母细胞核成熟效率无显著影响（P＞0.05），但高浓度处理组（1 600 nmol/L）显著降低卵母细胞早期凋亡率（P＜0.05），100和800 nmol/L的AL-8810可显著提高体外胚胎16-细胞期的发育效率（P＜0.05），800 nmol/L组桑葚胚形成率最高。以上研究结果表明：抑制前列腺素PGF2α信号可有效提高水牛卵母细胞体外成熟质量与早期胚胎发育潜力。
The purpose of this study was to explore the effect of PGF2α signal on the oocyte and early embryo in buffalo, the expression patterns of PTGFR in buffalo oocytes and early embryos development were determined by immunofluorescence and fluorescence quantification. Subsequently, the effects of AL-8810 on the maturation efficiency of oocytes and the efficiency of embryonic development in buffalo were studied by adding different concentrations of AL-8810 into the oocyte maturation medium and embryonic development medium. The results showed that PTGFR protein was widely distributed in buffalo oocytes and embryos, and the expression level of PTGFR gene was significantly higher in 4-16 cell stage than in unfertilized oocytes and morulae (P＜0.01). AL-8810 had no significant differences effect on the nuclear maturation efficiency of buffalo oocytes (P＞0.05), however, high concentration treatment group(1 600 nmol/L) significantly reduced the early apoptosis rate of buffalo oocytes (P＜0.05). AL-8810 at the concentration of 100 and 800 nmol/L significantly improved embryonic development efficiency in 16 cell stage, and the morula formation rate was the highest in the 800 nmol/L group. The above results indicated that inhibition of prostaglandin PGF2α signal effectively improved in vitro maturation quality of oocytes and early embryos development potential in buffalo.