为研究高致病性猪繁殖与呼吸综合征病毒(Highly Pathogenic Porcine reproductive and respiratory syndrome virus,HP-PRRSV)感染后血液性状的变化规律,本研究以大白猪×通城猪高代横交群体为对象,选用72头5周龄健康仔猪进行HP-PRRSV人工感染,在感染前和感染后第4,7,11,14,21,28,35天进行4种血常规指标的测定：白细胞计数、淋巴细胞百分比、单核细胞计数、粒细胞百分比,并与病毒载量进行相关分析。结果表明：感染后第4,7天与感染前相比,白细胞计数、淋巴细胞百分比、单核细胞计数均极显著降低(P<0.01),粒细胞百分比极显著升高(P<0.01)；感染后其它时间点与感染前相比均无显著变化。根据病毒载量和个体存活力将试验猪分为抗病和易感两组,发现抗病组和易感组的淋巴细胞百分比和粒细胞百分比在感染后第7天存在显著差异,其中抗病组的淋巴细胞百分比显著高于易感组(P<0.05),粒细胞百分比显著低于易感组(P<0.05)；其它时间点及其它指标均无显著差异。通过对试验猪感染后各时间点的血液指标和病毒载量进行相关分析,发现4种血液性状与病毒载量均存在中度相关(P<0.01)。研究结果对于进一步认识血液性状在PRRSV感染中的变化规律,以及与病毒载量的关系提供了基础资料。
To investigate the changes of hematological traits after infection with highly pathogenic porcine reproductive and respiratory syndrome virus, a total of 72 Large White×Tongcheng advanced generation inter-cross piglets of 5 weeks old ,infected with HP-PRRSV, were used for the artificial infected experiment. The WBC count, the lymphocyte percentage, the monocyte count and the granulocyte percentage were determined at 0,4,7,11,14,21,28,35 days post infection (DPI) ,then were correlated with viremia respectively. The results showed when compared to 0DPI, the WBC count, the lymphocyte percentage and the monocyte count were significantly reduced (P <0.01), and the granulocyte percentage was significantly increased (P <0.01) at both 4DPI and 7DPI, but there were no significant differences for the other DPI. Groups of piglets with PRRS-resistant and PRRS-susceptible were set on the basis of viremia and survive. It was detected that the lymphocyte and the granulocyte percentage were significantly different at 7DPI between groups. The lymphocyte percentage was significantly higher than PRRS-susceptible group(P <0.05), and the granulocyte percentage was significantly lower (P <0.05).For other DPI, as well as other hematological traits, there were no significant differences. Furthermore, the correlation analysis of hematological traits and viremia at all DPI in piglets revealed medium correlations between the hematological traits and viremia (P <0.01). Our current study provides basic data for further research on the changes of hematological traits after PRRSV infection and its relationship with viremia deeply.