A pathogenic bacterium was isolated from diseased Procambarus clarkii in Qianjiang, Hubei province. The strain was identified by physiological and biochemical assay, 16s rRNA sequence determination and phylogenetic analysis. Then the susceptibility to antibiotics was performed by agar diffusion method, the minimum inhibitory concentrations (MICs) were evaluated by micro-dilution method. The results showed that the physiological and biochemical characteristics similar to Klebsiella pheumonia. The 16s rRNA sequencing and phylogenetic analysis showed that the strain had close genetic relationship shared over 99% homologies with Klebsiella pheumonia. Therefore, the strain was identified as Klebsiella pheumonia. According to the results of susceptibility results, the strain was sensitive to cefotaxime and aerosporin among all the tested antibiotics These results indicated that the strain was a multi-resistant Klebsiella pheumonia strain.