黄颡鱼朗格汉斯细胞的鉴定及功能基因的表达分析
DOI:
作者:
作者单位:

华中农业大学

作者简介:

通讯作者:

中图分类号:

基金项目:

国家自然科学基金项目(面上项目,重点项目,重大项目)


The identification of Langerhans cell and the expression levels of gene-relatived to funcation of Langerhans cell in yellow catfish (Pelteobagrus fulvidraco)
Author:
Affiliation:

huazhong agricultural university

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    朗格汉斯细胞作为树突状细胞的亚型之一,主要分布于哺乳动物皮肤表皮和部分粘膜组织中,在抗细菌感染过程中发挥重要作用。为研究黄颡鱼朗格汉斯细胞的结构特点以及细菌感染对其功能相关基因表达的影响,采用免疫组化技术在黄颡鱼头肾和脾脏中检测到CD207阳性细胞,并通过透射电镜进一步观察到黄颡鱼朗格汉斯细胞的超微结构。荧光定量PCR结果表明,鲶爱德华氏菌能够诱导黄颡鱼免疫器官内朗格汉斯细胞功能相关基因的显著上调。上述结果表明,黄颡鱼朗格汉斯细胞的结构与其他脊椎动物类似,并且参与了机体抗鲶爱德华氏菌诱导的免疫反应。本试验不仅有助于我们分析朗格汉斯细胞在脊椎动物中的进化特点,更为黄颡鱼适应性免疫系统的研究提供一定的理论基础。

    Abstract:

    Langerhans cells, one of the subtypes of Dendritic cells, are mainly distributed in mammalian skin epidermis and some mucosal tissues, and play an important role in against bacterial infection. In order to study the structural characteristics of Langerhans cells and the effect of bacterial infection on the expression of gene-related the function of Langerhans cells, CD207 positive cells were detected in the head kidney and spleen of yellow catfish by immunohistochemistry, and the ultrastructure of Langerhans cells was further observed by transmissionSelectronSmicroscope. The results of real-time quantitative PCR showed that Edwardsiella ictaluri could induce significant up-regulation of gene-related the function of Langerhans cells in the immune organs of yellow catfish. The above results indicated that the structure of yellow catfish Langerhans cells was similar to that in other vertebrates and participate in the immune response induced by E.ictaluri. This study will not only help us analyze the evolutionary characteristics of Langerhans cells in vertebrates, but provides a theoretical basis for the research of the adaptive immune system of yellow catfish.

    参考文献
    相似文献
    引证文献
引用本文
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2020-01-09
  • 最后修改日期:2020-06-05
  • 录用日期:2020-10-30
  • 在线发布日期:
  • 出版日期: