为探究端粒酶逆转录酶TERT (Telomerase Reverse Transcriptase)基因在昆虫生长发育过程中的作用机制，本研究以萝卜蚜Lipaphis erysimi (Kaltenbach)为研究对象克隆了其TERT基因并分析了时空和时间表达情况。结果发现，萝卜蚜TERT基因的CDS区，长2 658 bp，能够编码885个氨基酸，其二级结构含41个α-螺旋，60个卷曲螺旋。系统进化分析表明，萝卜蚜TERT基因与麦双尾蚜Diuraphis noxia关系最近，与桃蚜Myzus persicae、豌豆蚜Acyrthosiphon pisum同源性较高。实时荧光定量PCR显示，TERT基因在萝卜蚜生长发育过程中的各个龄期及组织中均有表达，且成虫TERT基因的相对表达量依次高于1龄、2龄、3龄、4龄若虫；TERT基因在萝卜蚜腹部表达量最高，头部次之，胸部表达量最低，但均低于整虫的表达量。因此TERT基因在萝卜蚜的生长发育过程中起重要作用，为进一步研究TERT基因在昆虫中的生理功能奠定了基础。
Telomerase has the function of maintaining telomere length, and its catalytic subunit TERT (Telomerase Reverse Transcriptase) is tightly regulated in higher organisms such as mammals, and is usually not expressed in somatic cells. However, in lower eukaryotes such as invertebrates, TERT is often expressed in somatic cells, but the physiological function is not clear. In order to clarify the physiological function of TERT, the TERT gene of Lipaphis erysimi (Kaltenbach) was cloned and analyzed for its spatial and temporal expression. The results showed that the CDS region of the radish TERT gene was 2658 bp and capable of encoding 885 amino acids. Its secondary structure contained 41 α-helices and 60 coiled coils. Phylogenetic analysis indicated that the Lipaphis erysimi TERT gene has the closest relationship with Diuraphis noxia, and has high homology with Myzus persicae and Acyrthosiphon pisum, but has low homology with other insects. Real-time quantitative PCR showed that the TERT gene was expressed in all ages and tissues during the growth and development of Lipaphis erysimi,and the relative expression of adult TERT gene was higher than that of 1st, 2nd, 3rd and 4th nymphs. The expression of TERT gene in the abdomen of the Lipaphis erysimi was the highest, followed by the head, and the lowest in the chest, but lower than the expression of the whole worm. This study laid the foundation for further study of the physiological functions of TERT in insects.