A vibration-filling scheme for soft-pot seedling of citrus was proposed and a vibration-filling production line capable of filling 36 plastic soft pots or four non-woven fabric large pots at a time was designed to address the issue of time-consuming and labor-intensive substrate filling operations for soft-pot seedling of citrus in China. Theoretical analysis and simulation calculation methods were used to design the structure and determine parameters of the key components of the substrate mixing and conveying unit， the filling unit and the tray conveying unit. The prototype was successfully developed and manufactured， and its performance and working efficiency were tested through filling operation experiments. The results showed that the production line run stably with excellent operational performance. There were no collapses or missing fills for both plastic soft pots and non-woven fabric large pots. The working efficiency of plastic soft pots and non-woven fabric large pots was 4 320 and 480 pots per hour， respectively， with high efficiency and good filling uniformity. It will provide a reference for the development and optimization of filling and transfer equipment for soft-pot seedling of citrus.