Brassica napus cultivar ‘Dadi 199’ was used to conduct a field plot experiment to establish a collaborative technical mode for high-yield rapeseed and efficient utilization of water-fertilizer in the rice-rapeseed rotation system in the middle and lower reaches of the Yangtze River.Four treatments including T1 （conventional cultivation without fertilization），T2 （conventional cultivation with conventional fertilization），T3 （conventional cultivation with formula fertilizer），and T4 （no-tillage and mulching combined with formula and organic fertilizers） were set up.The effects of different treatments on the photosynthetic parameters，yield，components of yield，nutrient accumulation，and water-fertilizer use efficiency were analyzed.Results showed that the net photosynthetic rate，SPAD value，leaf area，and instantaneous water use efficiency in T4 was enhanced compared to that in T2 and T3，with a significant difference between T4 and T2 （P<0.05）.T4 achieved a 20.5% and 12.0% increase in yield compared to that in T2 and T3 mainly by increasing the siliques per plant，seeds per silique，silique length and 1 000-seed weight.Among different treatments，T4 had the highest accumulation and proportion of nitrogen，phosphorus，and potassium nutrients in seeds，but the accumulation of phosphorus and potassium in the roots，stems，and silique walls was lower than that in T2.The contribution rate，agronomic utilization rate，partial productivity，physiological utilization rate of fertilizer，and the use rate of water followed the order of T4>T3>T2，with an increase of 16.5%，53.1%，31.2%，48.7%，and 20.7% in T4，respectively，compared to that in T2.There was a significant difference between T4 and T2，except for the contribution rate of fertilizer.The water-fertilizer utilization efficiency in T3 was better than that in T2，with no significant difference between the two.It is indicated that T4 （no-tillage and mulching combined with formula and organic fertilizers） for direct-seeded rapeseed is a planting mode suitable for promotion in the middle and lower reaches of the Yangtze River region to achieve the high-yield and efficient utilization of water-fertilizer of rapeseed.