The flue-cured tobacco Yunyan 87 was used to study the physicochemical properties of tobacco planting soil and the response of the growth， development of tobacco， and the quality of tobacco leaves to different types of biochar. The effects of different types of biochar on the growth， development of tobacco， and the quality of tobacco leaves in Southern Anhui were identified by setting up treatments including conventional fertilization （CK）， conventional fertilization + pyrolysis biochar （T1）， conventional fertilization + plant-based biochar （T2）， conventional fertilization + animal-based biochar （T3）， and conventional fertilization + mixed types of biochar （T4）. The results showed that T2 significantly increased the content of available phosphorus and potassium in the soil by 26.80% and 17.84%， respectively. T3 had the best coordination of chemical composition in tobacco leaves， with sugar-alkaloid balance and potassium-chloride balance of 13.53 and 4.81， respectively. Based on the contribution rate of biochar to the yield and output value of tobacco，T4 increased the output value per hectare by 23.60%. The results showed that the application of biochar can promote tobacco plant development， improve soil physical and chemical properties， and enhance tobacco leaf growth； plant-based biochar has a more significant impact on physical and chemical properties， while animal-based biochar exhibits the best effect in promoting tobacco growth and development as well as improving tobacco leaf quality. In production practice， appropriate carbon source biochar can be added according to actual needs to improve soil conditions， and further promote tobacco growth and development and enhance tobacco leaf quality.