The pot flooding method was used to study the waterlogging tolerance of grapevine to mine and utilize the plant rhizobacteria and their functions. Cuttings of 1-year-old Kyoho grapevine were subjected to flooding stress for 21 days and recovery for 7 days after flooding. 16S rRNA high-throughput sequencing technology was used to sequence bacteria in soil， analyze the composition and structure of bacterial community， and study the effects of flooding stress on the composition of rhizobacteria community in Kyoho grapevine. The results showed that the abundance of rhizobacteria in Kyoho grapevine was the highest after 21 days of flooding， and the diversity of rhizobacteria in Kyoho grapevine was the highest after 14 days of flooding. The relative abundance of Thiobacillus increased significantly during the recovery period after stress. The results of functional prediction of root bacteria showed that there were significant changes in the metabolism of lipid， terpenoid and polyketide. It is indicated that short-term flooding stress promotes the production of metabolites in plants to change bacterial communities in response to stress， but prolonged flooding disrupts plant metabolism， leading to an increase in the abundance of potentially harmful bacterial flora and a decrease in beneficial bacterial flora in plants. It will provide theoretical basis for the mining and utilization of rhizobacteria and their functions.