金针菇中免疫调节蛋白编码基因FIP-fve的功能
作者:
作者单位:

1.沈阳农业大学生物科学技术学院,沈阳110866;2.辽宁省林业科学研究院,沈阳110032

作者简介:

通讯作者:

林景卫,E-mail: 2009500003@syau.edu.cn

中图分类号:

S567.3+9

基金项目:

辽宁省教育厅科学研究基础项目(LSNQN202013)陈焕,E-mail:2507297357@qq.com


Native bio-function of FIP-fve towards Flammulina filiformis
Author:
Affiliation:

1.College of Biological Science and Technology, Shenyang Agricultural University, Shenyang 110866,China;2.Liaoning Academy of Forestry Sciences,Shenyang 110032,China

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    摘要:

    为探究FIP-fve基因在金针菇本体的生物学功能,构建了pCAMBIA1301-pGPD-FIP-fve超表达载体并将其转化至农杆菌LBA4404中,采用农杆菌介导的金针菇转化法,最终获得了FIP-fve基因超表达的金针菇;进行FIP-ve基因沉默(RNAi: FIP-fve)、FIP-fve超表达(pGPD: FIP-fve)和野生金针菇(CK)菌丝体培养和子实体栽培,并记录菌丝体和子实体时期的生物学性状。结果显示:pGPD: FIP-fve金针菇菌丝生长速度最快,CK金针菇次之,而RNAi: FIP-fve金针菇生长速度最为缓慢;子实体出菇栽培后,在子实体数量、菌柄长度、子实体产量和生物学效率方面,pGPD: FIP-fve金针菇都明显优于CK金针菇,而RNAi: FIP-fve金针菇的子实体生长状况最差。研究结果表明,FIP-fve基因能够促进金针菇菌丝体的生长,并对金针菇原基形成具有促进作用,最终影响金针菇的产量。

    Abstract:

    FIP-fve is a low molecular-mass protein identified from Flammulina filiformis, which has many biological activities including immunomodulation. Our previous studies found that FIP-fve had certain growth-promoting effect on F. filiformis mycelia. We constructed an over-expression vector pCAMBIA1301-pGPD-FIP-fve, and transformed it into Agrobacterium tumefaciens LBA4404 strain to study the native biological function of FIP-fve to F. filiformis. FIP-fve gene overexpressed F. filiformis was obtained via Agrobacterium transformation. By comparing the biological characteristics of FIP-fve gene-silent F. filiformis (RNAi:FIP-fve), FIP-fve gene-overexpressed F. filiformis (pGPD:FIP-fve) and wild F. filiformis (CK) in mycelium and fruiting body stages, we found that the growth speed of pGPD:FIP-fve was faster than that of CK, whereas the growth speed of RNAi:FIP-fve was the lowest. After cultivating the fruiting body, yield-related traits of pGPD:FIP-fve were significantly better than that of CK in terms of fruiting body numbers, stalk length, fruiting body yield and biological efficiency, and those traits of RNAi: FIP fve were the worst. It is indicated that FIP-fve significantly promote the growth of F. filiformis mycelia and affect the final yields of F. filiformis.

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陈焕,范天宁,白玉东,郭芡芡,王浩安,陈罡,高英旭,林景卫.金针菇中免疫调节蛋白编码基因FIP-fve的功能[J].华中农业大学学报,2023,42(2):131-138

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  • 收稿日期:2022-08-03
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  • 在线发布日期: 2023-03-31
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