To identify the dominant strains in the intestinal tract in female adults of the quarantine fruit fly pest， Zeugodacus tau （Walker）， and their effects on feeding ability， nutritional status and fecundity， here traditional microorganism isolation and molecular identification techniques were use to isolate and identify the culturable bacteria， and then antibiotic treatment and intestinal dominant strains supplementation were carried out to detect the intake， hemolymph amino acid content and egg production of Z. tau.The results indicated that the elimination of intestinal bacteria significantly reduced the oviposition， food intake and hemolymph amino acid content of female adult fruit flies to 34.9%， 43.2% and 60.8% of the control （without antibiotic treatment） levels.Subsequently， 9 cultivable strains were isolated and identified as Proteobacteria and Firmicutes， and are mainly distributed in the genera of Enterococcus， Enterobacter and Bacillus.The dominant strains were Enterococcus sp.ZYY 2-97 （57.41%） and Bacillus sp.ZYY 1-83 （17.59%）.In addition， after supplemented with ZYY 2-97 and ZYY 1-83 strains， the total fecundity of the female adults were significantly recovered to 75.00% and 86.98% of the control level， and the food intake were significantly recovered to 117.53% and 117.85% of the control level， respectively.Meanwhile， the amino acids contents in hemolymph were recovered to 94.80% and 87.81% of the control level.Our findings demonstrated that the intestinal bacteria could affect the fecundity of Z. tau by regulating the feeding capacity and nutritional status of the female adults， and the dominant culturable strains play a key role in this process.