The genetic diversity of Hylocereus germplasm resources was analyzed and evaluated to provide a scientific basis for the innovative utilization of Hylocereus germplasm resources.14 qualitative traits and 9 quantitative traits in 58 Hylocereus germplasm were observed and described.The phenotypic traits were evaluated by variation analysis，index of diversity，correlation analysis.The results showed that most of the traits in the 58 tested germplasm had the characteristics of high degree of variation and rich types.The diversity index（H′） of qualitative traits was 0.234-1.809.The variation range of 9 quantitative traits was large，and the coefficient of variation was 11.25%-35.32%，among which the coefficient of variation of Vc content was the largest.The results of correlation analyses showed that most of the quantitative traits had significant or extremely significant correlations.It is indicated that there are real genetic differences among the 58 Hylocereus germplasm resources，and the genetic diversity is rich.