Control effects of six different treatments on common scab of minituber cultured in vermiculite were evaluated through field experiment,and high-through sequencing technology of 16S ribosomal genes was employed to studied changes in community structure of bacteria. The results showed that the treatment of dazomet+Penicillium 34107 had the best control effect (93.43%),which was significantly greater than the Zhongshengmycin control (65.10%),and had the highest amount of commercial minitubers and economic profit of 3.483×106tuber/hm2and ￥3.570×105/hm2,repsectively. The relative abundance of Proteobacteria,Actinobacteria and Bacteroidetes were the highest in all treatments,but varied in a greater range among different treatments. Except for the treatment of Penicllium 34170，the diversity indexes of bacteria of all the others decreased,however,that of dazomet+Penicillium 34107 was higher than Zhongshengmycin and significantly higher than dazomet (P<0.05). Statistic analysis demonstrated that a total of 185 genera had significantt changes in abundance (P<0.05),of which 92 (49.7%)were common in all treatments. The treatments of dazomet and Penicillium 34107 had the largest number of significantly changed genera (112),while dazomet+Pencillium 34107 had the smallest number of only 30 genera that were signficnatly increased. The abundance of the benificial bacterium (Pseudomonas) was significantly increased in the treatments of both Pencicillium 34107 and dazomet+Penicillium 34107,indicating that Penicillium 34107 applied individiually,or especially with dazomet can promote progation of benifical bacteria in vermiculite. Correlation analysis showed that Streptomyces was significantly positively correlated with 55 genera (P<0.05,r>0.6),including Mesorhizobium,Solirubrobacter,and Asticcacaulis,et al,while negatively correlated with 8 genera (P<0.05,r＜-0.6),including Dyadobacter,Pedobacter,Paenarthrobacter,et al. The above results illumstrated that soil disinfection combined with Penicillium 34107 had the best control effect on common scab of minituber,and meantime,improved the bacterial diversity and promoted benificial bacteria. In addtion, Streptomyces was closely correlated with multiple kinds of soil bacteria. These results provide a good method and theoretical basis for the control of common scab of minituber.