The effects of soil acidification on soil nutrient availability and plants nutrient uptake,accumulation and distribution were investigated to provide a basis for soil acidification treatment,especially nutrient efficient utilization. Cherry tomatoes (Lycopersivon esculentum Mill) was used to conduct pot experiment with acidic red soil (pH 5.05). The soil pH was adjusted to 4.0,5.0,6.0 and 7.0 with citric acidsodium hydrogen phosphate buffer and sodium carbonate-sodium bicarbonate buffer. There were 4 treatments with 5 replicates per treatment in the experiment. The results showed that measured soil pH were 4.35,4.92,5.89,6.85,adjusted down by 0.70,0.13 and increased by 0.84 and 1.80 pH unit,respectively. Soil acidification significantly reduced the leaf area and biomass of cherry tomato,especially the root biomass,and root to shoot ratio. Soil acidification severely inhibited the accumulation of N,P,K,Mg and Fe in roots,promoted the accumulation and distribution of Fe and Mn in stem,and Ca and Mg in leaves. Soil acidification significantly decreased accumulation of N,P,K,Ca,Mg and Fe in roots. More N,K,Fe and Mn was accumulated in stems,more P,Ca and Mg was accumulated in leaves. Particularly,Mn concentration in all parts of plants was raised significantly. It is indicated that the root is the most severe and sensitive part of whole tomato plant inhibited by soil acidification and most nutrients are absorbed by the roots. Soil acidification blocked migration and reduced accumulation significantly.