In this experiment,at 18℃ and 2 ℃,enrofloxacin was injected at a dose of 20 μg/kg to study the pharmacokinetics characteristics and residues of enrofloxacin and ciprofloxacin in Procambarus clarkii.The results showed that the recovery rates of enrofloxacin and ciprofloxacin in the hemolymph,muscle,hepatopancreas and pancreas were from 80% to 110%.The intra day variation coefficients of the detection values were less than 5%,and the interday variation coefficients were less than 6%.The detection limits were all below 0.01 μg/mL (μg/g).The Tmax of enrofloxacin in the hemolymph,muscle,hepatopancreas at 18℃ was 0.083 0 h,0.344 7 h,and 1.933 5 h,respectively,and the Tmax of ciprofloxacin was 0.083 0 h,0.263 4 h,and 1.165 8 h,respectively.Increasing the temperature accelerated the absorption of enrofloxacin in P.clacherii.The order of elimination halflife T1/2β was hepatopancreas (55.740 3 h)> muscle (52.743 7 h)> plasma (18.608 7 h).With enrofloxacin and ciprofloxacin as residual markers,the elimination period of enrofloxacin in the hepatopancreas and pancreas was 311.47 h at 25℃,and the elimination period of enrofloxacin in hepatopancreas and pancreas was 417.77 h at 18℃.It is recommended that the enrofloxacin withdrawal period is 325 (℃·d).