In order to find the morphological differences between hybrid 1 (Misgurnus anguillicaudatus♀×Paramisgurnus dabryanus♂),hybrid 2 (Paramisgurnus dabryanus♀×Misgurnus anguillicaudatus♂) and their parents，the morphological differences,countable traits and measurable traits were analyzed by combining morphological measurements with statistical analysis．The results showed that the two hybrids were similar in appearance,integrating their parental characteristics. For example,the welldeveloped caudal fold at the caudal peduncle of the two hybrids was similar to P. dabryanus,while the pattern on the body was more similar to that of M. anguillicaudatus,most of which were round spots with a small number of stripes. The body shape of hybrids was between their parents，which was rounder than P. dabryanus. Cluster analysis and principal component analysis indicated that the morphology of the two hybrids were similar to each other,and were more similar to P. dabryanus．The comprehensive accuracy of discriminant analysis reached 94.1%,which can effectively identify the two loach and their hybrids. There was no significant difference of hatching rate,deformity rate and early survival rate between the hybrids and selfcrossing group (P>0.05).