Clarifying the mechanism, path and effect of Agricultural Mechanization on Farmers’ income in different production links is of great significance to promote the optimization of agricultural machinery and equipment structure and increase farmers’ income. Based on agricultural bicycle theory and intermediary effect theory, this paper selects the panel data of 25 major agricultural provinces in China from 2008 to 2019, constructs a dynamic panel model and intermediary effect model to test the proposition hypothesis, and explores the intermediary role of agricultural product price, labor transfer, agricultural operation scale and food production efficiency in the process of agricultural mechanization affecting farmers’ income. The research shows that the development of agricultural mechanization not only promotes the increase of farmers’ income, but also plays a significant role in narrowing the income gap between urban and rural areas; Agricultural Mechanization mainly affects farmers’ income through the intermediary path of labor transfer and agricultural product price. Agricultural mechanization mainly affects farmers’ income through the intermediary path of labor transfer and agricultural product price. Among them, agricultural machinery in the production link reduces farmers’ agricultural income by inhibiting the transfer of labor force; Agricultural machinery in processing links can promote farmers’ agricultural income by promoting labor transfer; Increase farmers’ agricultural income by raising the price of agricultural products. It is proposed that adjusting the structure of agricultural machinery and equipment, increasing agricultural machinery subsidies in production and strengthening the popularization of agricultural machinery technology in processing are important ways to effectively promote high quality and efficiency of agriculture.